Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress

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• 作者：Xituan Ji ; MD^a ; ¹ ; Ye Tian ; MD^b ; ¹ ; Keliang Xie ; PhD ; MD^c ; ¹ ; Weiping Liu ; PhD ; MD^a ; Yan Qu ; MD^a ; Zhou Fei ; PhD ; MD^a ; ^{Tougaozy2011@126.com} ; ^{feizhou@fmmu.edu.cn}
• 关键词：Traumatic brain injury (TBI) ; Reactive oxygen species (ROS) ; Oxidative stress ; Hydrogen-rich saline (HS)
• 刊名：Journal of Surgical Research
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：178
• 期：1
• 页码：e9-e16
• 全文大小：715 K

文摘

Background

Hydrogen gas (H₂) has been considered as a novel antioxidant to selectively reduce the toxic reactive oxygen species (ROS) such as hydroxyl radical (?OH) without affecting the other signal ROS. Our recent study shows that H₂ inhalation is beneficial to traumatic brain injury (TBI) via reducing oxidative stress. In contrast to H₂, hydrogen-rich saline (HS) may be more suitable for clinical application. The present study was designed to investigate whether HS has a protective effect against TBI via reducing oxidative stress in rats.

Methods

TBI model was induced by controlled cortical impact injury. Different dosages of HS were intraperitoneally administered at 5 min after TBI operation. We then measured the brain edema, blood-brain barrier (BBB) breakdown, neurological dysfunction and injury volume in all animals. In addition, the oxidative products and antioxidant enzymes in brain tissues were detected.

Results

TBI-challenged rats exhibited significant brain injuries characterized by the increase of BBB permeability, brain edema, and lesion volume as well as neurological dysfunction, which were dose-dependently ameliorated by HS treatment. Moreover, we found that HS treatment increased the endogenous antioxidant enzymatic activities and decreased the oxidative product levels in brain tissues of TBI-challenged rats.

Conclusion

Hydrogen-rich saline can exert a protective effect against TBI via reducing oxidative stress. Molecular hydrogen may be a more effective therapeutic strategy for TBI patients.