Reduction in renal blood flow following administration of norepinephrine and phenylephrine in septic rats treated with K_ir6.1 ATP-sensitive and K_Ca1.1 calcium-activated K⁺ channel blockers

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• 作者：Bruna da Rosa Maggi Sant&rsquo ; Helena^a ; Karla L. Guarido^b ; Priscila de Souza^a ; Sandra Crestani^b ; J. Eduardo da Silva-Santos^b ; ^{j.e.silva.santos@ufsc.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Phenylephrine hydrochloride (PubChem CID ; 5284443) ; (S)-(+)-Norepinephrine l-bitartrate (PubChem CID ; 168929) ; Tetraethylammonium chloride (PubChem CID ; 5946) ; Glibenclamide (PubChem CID ; 3488) ; Iberiotoxin (PubChem CID ; 16132435) ; Minoxidil sulfate (PubChem CID ; 4202) ; NS 1619 (PubChem CID ; 4552) ; Potassium chloride (PubChem CID ; 4873) ; Sodium chloride (PubChem CID ; 5234) ; Calcium chloride (PubChem CID ; 6093260) ; Potassium dihydrogen phosphate (PubChem CID ; 516951) ; Magnesium sulfate (PubChem C
• 刊名：European Journal of Pharmacology
• 出版年：2015
• 出版时间：15 October 2015
• 年：2015
• 卷：765
• 期：Complete
• 页码：42-50
• 全文大小：1346 K

文摘

We evaluated the effects of K⁺ channel blockers in the vascular reactivity of in vitro perfused kidneys, as well as on the influence of vasoactive agents in the renal blood flow of rats subjected to the cecal ligation and puncture (CLP) model of sepsis. Both norepinephrine and phenylephrine had the ability to increase the vascular perfusion pressure reduced in kidneys of rats subjected to CLP at 18 h and 36 h before the experiments. The non-selective K⁺ channel blocker tetraethylammonium, but not the K_ir6.1 blocker glibenclamide, normalized the effects of phenylephrine in kidneys from the CLP 18 h group. Systemic administration of tetraethylammonium, glibenclamide, or the K_Ca1.1 blocker iberiotoxin, did not change the renal blood flow in control or septic rats. Norepinephrine or phenylephrine also had no influence on the renal blood flow of septic animals, but its injection in rats from the CLP 18 h group previously treated with either glibenclamide or iberiotoxin resulted in an exacerbated reduction in the renal blood flow. These results suggest an abnormal functionality of K⁺ channels in the renal vascular bed in sepsis, and that the blockage of different subtypes of K⁺ channels may be deleterious for blood perfusion in kidneys, mainly when associated with vasoactive drugs.