A strategy for soluble overexpression and biochemical characterization of halo-thermotolerant Bacillus laccase in modified E. coli
详细信息 查看全文
- 作者：Azam Safarya ; c ; Rezvan Moniria ; b ; moniri@kaums.ac.ir" class="auth_mail" title="E-mail the corresponding author ; moniri_re@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Maryam Hamzeh-Mivehroudc ; d ; Siavoush Dastmalchic ; d ; dastmalchi.s@tbzmed.ac.ir" class="auth_mail" title="E-mail the corresponding author ; siavoush11@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Syringaldazine (PubChem CID ; 5379425) ; 2 ; 2&prime ; -Azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (PubChem CID ; 5464076) ; Sodium Chloride (PubChem CID ; 5234) ; EDTA (PubChem CID ; 6049) ; SDS (PubChem CID ; 3423265) ; NaN3 (PubChem CID ; 33557)
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 June 2016
- 年：2016
- 卷：227
- 期：Complete
- 页码：56-63
- 全文大小：1247 K
文摘
- •
Laccase gene from newly isolated Bacillus sp. SL-1 was cloned and overexpressed.
- •
A method was introduced for soluble expression of laccase in Origami strain.
- •
The laccase activity under different oxygen availability was investigated.
- •
The produced laccase retained 50% activity after one hour incubation at 70 °C.
- •
The enzyme was resistant to various inhibitors and organic solvents.