Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene

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• 作者：Heema Hewitson ; Victoria Wood ; Neli Kadeva ; Glenda Cornwell ; Stefano Codognotto ; Emma Stephenson ; Dusko Ilic ; ^{dusko.ilic@kcl.ac.uk" class="auth_mail" title="E-mail the corresponding author}
• 刊名：Stem Cell Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：16
• 期：2
• 页码：256-258
• 全文大小：642 K

文摘

The KCL025 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation in the NF1 gene encoding neurofibromin (c.3739–3742 ΔTTTG). Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.