Several authors have suggested that nitrogen-based fertilizers may be contributing to the global amphibian decline. We have studied the impact of sodium nitrite on early aquatic stages of
Epidalea calamita,
Pelophylax perezi and
Hyla meridionalis larvae from Doñana National Park (coastal wetland) and
P. perezi from Gredos Mountain (high mountain ponds), exposed during 10 to 16 days.
After 8 days of exposure all P. perezi larvae from Doñana presented 100 % mortality at 5 mg l−1 ![]()
while E. calamita larvae mortality rates were significantly lower at that concentration after 15 days. However, for H. meridionalis at day 15 no deaths were registered at 5 mg l−1 0b366"">![]()
and at 20 mg l−1 ![]()
presented intermediate mortality rates. In Doñana the 10 d LC50 of older H. meridionalis larvae was between 20 and 30 mg l−1 ![]()
whilst for P. perezi it was below 5 mg l−1 90bde95057c8f3a80d858"">![]()
. These results indicate inter-specific variation of the sensitivity of larval amphibians to nitrite.
Gredos Mountain P. perezi larvae exposed since the egg stage were highly sensitive to nitrite, with a 16 d LC50 below 0.5 mg l−1 ![]()
. The same species in Doñana had a 15 d LC50 between 5 and mg l−1 ![]()
. These results suggest that there is also intra-specific variation in sensitivity of amphibian larvae to nitrite: mountain amphibian populations appear to be more sensitive to polluted environments than coastal populations. Geographic and genetic variation and evolutionary adaptation of tolerance may also be the keys to variation amongst populations of the same species.
