Vγ9Vδ2 T cells recovered from eyes of patients with Behçet's disease recognize non-peptide prenyl pyrophosphate antigens

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• 作者：Verjans ; Georges M.G.M. ; Martin van Hagen ; P. ; van der Kooi ; Alexander ; Osterhaus ; Albert D.M.E. ; et. al.
• 关键词：Behç ; et's disease ; Uveitis ; Intra-ocular Vγ ; 9V&#948 ; 2 T cells ; Heat shock protein ; Prenylpyrophosphates ; BD ; Behç ; et's disease ; TCL ; T cell line ; IOF ; intra-ocular fluid ; HSP65 ; heat shock protein 65 kDa ; PPP ; prenylpyrophosphate ; IPP ; iso
• 刊名：Journal of Neuroimmunology
• 出版年：2002
• 出版时间：September, 2002
• 年：2002
• 卷：130
• 期：1-2
• 页码：46-54
• 全文大小：263 K

文摘

The phenotype and antigen-specificity of T cells expanded by mitogenic stimulation from intra-ocular fluid (IOF) samples of affected eyes of six Behçet's disease (BD) patients, and seven patients with other uveitis entities, were determined. High numbers of γδ T cells, predominantly Vγ9Vδ2 T cells, were only detected in the IOF-derived TCL of three BD patients. Whereas no TCL responded to heat shock protein (HSP) 65 kDa, reactivity to isopentyl pyrophosphate (IPP) and related non-peptide prenyl pyrophosphates (PPP) was restricted to the γδ T cell containing TCL. Upon IPP stimulation, these TCL secreted IFN-γ but no IL-4. By single-cell analysis of intracellular IFN-γ production and CD69 expression the IOF-derived IPP-specific T cells were identified as CD4⁻CD8⁻ γδ T cells. The data presented suggest the infiltration of PPP-specific Vγ9Vδ2 Th1-like cells into the eye of BD patients with uveitis.