Molecular detection of tick-borne protozoan parasites in a population of domestic cats in midwestern Brazil

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• 作者：Í ; sis Assis Braga^a ; ^b ; Dirceu Guilherme de Souza Ramos^a ; ^c ; Arlei Marcili^d ; ^e ; André ; ia Lima Tomé ; Melo^a ; ^f ; Isis Indaiara Gonç ; alves Granjeiro Taques^a ; ^g ; Alexandre Mendes Amude^f ; Cristiane Silva Chitarra^a ; ^g ; Luciano Nakazato^g ; Valé ; ria Dutra^g ; Richard de Campos Pacheco^g ; Daniel Moura Aguiar^g ; ^{danmoura@ufmt.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Feline hemoparasites ; Hepatozoon spp. ; Cytauxzoon spp. ; Babesia spp.
• 刊名：Ticks and Tick-borne Diseases
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：7
• 期：5
• 页码：1004-1009
• 全文大小：878 K

文摘

Some tick-borne pathogens that infect domestic cats have been considered emergent in veterinary medicine. Occurrences of Hepatozoon spp., Babesia spp. and Cytauxzoon spp. have been described in several regions of Brazil. This paper offers a comprehensive analysis of the 18S rRNA gene of a Hepatozoon sp. strain detected in domestic cats in the metropolitan area of Cuiabá, in Midwestern Brazil. Based on a molecular analysis, we detected the presence of Hepatozoon species circulating among cats in this region. The aforementioned strain is closely related to other isolates of H. felis detected in wild felids. Moreover, a phylogenetic analysis indicates that this genotype is grouped into a clade of 18S rRNA sequences previously described for the genus Hepatozoon in wild felids around the world. Hepatozoon felis strains detected in cats from Spain and Israel showed, respectively, 98% and 97% identity to our sequence and are clustered on a separate branch of the phylogenetic tree. This finding suggests a high diversity of Hepatozoon genotypes occurring in cats in Europe and South America. None of the analyzed cats were positive for Babesia spp. or Cytauxzoon spp. by PCR analysis.