Expression of human β-N-acetylhexosaminidase B in yeast eases the search for selective inhibitors
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- 作者：Jana Krejzová ; a ; b ; janakrejza@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Natallia Kulikc ; kulik@nh.cas.cz" class="auth_mail" title="E-mail the corresponding author ; Kristý ; na Slá ; mová ; a ; slamova@biomed.cas.cz" class="auth_mail" title="E-mail the corresponding author ; slamova.kristyna@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Vladimí ; r Křena ; kren@biomed.cas.cz" class="auth_mail" title="E-mail the corresponding author
- 关键词：β-N-acetylhexosaminidase ; Hex B ; Enzyme inhibition ; O-GlcNAcase ; Pichia pastoris
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：89
- 期：Complete
- 页码：1-6
- 全文大小：652 K
文摘
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Human lysosomal β-N-acetylhexosaminidase Hex B was expressed in Pichia pastoris.
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Method for a facile, robust and cost-effective expression of Hex B was developed.
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Obtained Hex B was utilized in the screening of potential inhibitors.
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Human O-GlcNAcase was also employed to assess selectivity of the inhibitors.