The nociceptin/orphanin FQ-like opioid peptide in nervous periesophageal ganglia of land snail Helix aspersa

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• 作者：Martha Leó ; n-Olea^a ; ^{marthalo@imp.edu.mx} ; ^{mlomx@yahoo.com.mx} ; Carolina Miller-Pé ; rez^a ; Eduardo S&aacute ; nchez-Islas^a ; José ; Mendoza-Sotelo^a ; René ; Garduñ ; o-Gutié ; rrez^a ; Patricia de Gortari^b ; Marí ; a Isabel Amaya^b
• 关键词：Anatomical distribution ; Neuropeptide ; Nociceptin ; Invertebrate ; Mollusc ; RT-PCR
• 刊名：Brain Research
• 出版年：2013
• 出版时间：10 April, 2013
• 年：2013
• 卷：1505
• 期：Complete
• 页码：22-46
• 全文大小：4996 K

文摘

The neuropeptide nociceptin/orphanin FQ (N/OFQ) and its receptor are members of the endogenous opioid peptide family. In mammals N/OFQ modulates a variety of biological functions such as nociception, food intake, endocrine, control of neurotransmitter release, among others. In the molluscs Cepea nemoralis and Helix aspersa the administration of N/OFQ produces a thermopronociceptive effect. However, little is known about its existence and anatomic distribution in invertebrates. The aim of this study was to provide a detailed anatomical distribution of N/OFQ like peptide immunoreactivity (N/OFQ-IL), to quantify the tissue content of this peptide, as well as to demostrate molecular evidence of N/OFQ mRNA in the nervous tissue of periesophageal ganglia of the land snail H. aspersa. Immunohistochemical, immunocytochemical, radioimmunoanalysis (RIA) and reverse transcription-polymerase chain reaction (RT-PCR) techniques were used. With regard to RT-PCR, the primers to detect expression of mRNA transcripts from H. aspersa were derived from the rat N/OFQ opioid peptide. We show a wide distribution of N/OFQ-IL in neurons and fibers in all perioesophageal ganglia, fibers of the neuropile, nerves, periganglionar connective tissue, aortic wall and neurohemal sinuses. The total amount of N/OFQ-IL in the perioesophageal ganglia (7.75¡À1.75 pmol/g of tissue) quantified by RIA was similar to that found in mouse hypothalamus (10.1¡À1.6 pmol/g of tissue). In this study, we present molecular evidence of N/OFQ mRNA expression. Some N/OFQ-IL neurons have been identified as neuroendocrine or involved in olfaction, hydro-electrolyte regulation, feeding, and thermonociception. Therefore, we suggest that N/OFQ may participate in these snail functions.