Photoactivated hypericin increases the expression of SOD-2 and makes MCF-7 cells resistant to photodynamic therapy

详细信息    查看全文

• 作者：Patrí ; cia Kim&aacute ; kov&aacute ; ^a ; Peter Sol&aacute ; r^a ; ^{peter.solar@upjs.sk} ; Barbora Feckov&aacute ; ^a ; Veronika Sačkov&aacute ; ^a ; Zuzana Sol&aacute ; rov&aacute ; ^b ; Lenka Ilkovičov&aacute ; ^a ; Martin Kello^b
• 关键词：Photodynamic therapy ; Hypericin ; SOD-2 ; MCF-7 ; Resistance
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：85
• 期：Complete
• 页码：749-755
• 全文大小：1521 K
• 卷排序：85

文摘

Photoactivated hypericin increased production of reactive oxygen species in human breast adenocarcinoma MCF-7 as well as in MDA-MB-231 cells 1 h after photodynamic therapy. On the other hand, reactive oxygen species dropped 3 h after photodynamic therapy with hypericin, but only in MCF-7 cells, whereas in MDA-MB-231 cells remained elevated. The difference in the dynamics of reactive oxygen species after hypericin activation was related to increased activity of SOD-2 in MCF-7 cells compared to MDA-MB-231 cells. Indeed, photodynamic therapy with hypericin significantly increased SOD-2 activity in MCF-7 cells, but only slightly in MDA-MB-231 cells. In this regard, SOD-2 activity correlated well with enhanced both mRNA expression as well as SOD-2 protein level in MCF-7 cells. The role of SOD-2 in the resistance of MCF-7 cells to photodynamic therapy with hypericin was monitored using SOD-2 inhibitor - 2-methoxyestradiol. Interestingly, the combination of photodynamic therapy with hypericin and methoxyestradiol sensitized MCF-7 cells to photodynamic therapy and significantly reduced its clonogenic ability. Furthermore, methoxyestradiol potentiated the activation of caspase 3/7 and apoptosis induced by photodynamic therapy with hypericin.