A phosphate-stimulated NAD(P)⁺-dependent glyceraldehyde-3-phosphate dehydrogenase in Bacillus cereus

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• 作者：Iddar ; Abdelghani ; Serrano ; Aurelio ; Soukri ; Abdelaziz
• 关键词：Glyceraldehyde-3-phosphate dehydrogenase ; NAD⁺ ; NADP⁺ ; Phosphate ; Soil bacterium ; Bacillus cereus
• 刊名：FEMS Microbiology Letters
• 出版年：2002
• 出版时间：May 21, 2002
• 年：2002
• 卷：211
• 期：1
• 页码：29-35
• 全文大小：235 K

文摘

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme of central carbon metabolism, was studied in a Bacillus cereus strain isolated from the phosphate layer from Morocco. Enzymatic assays with cell extracts demonstrated that when grown on Luria–Bertani (LB) medium, B. cereus contains a major NAD⁺-dependent GAPDH activity and only traces of NADP⁺-dependent activity, but in cells grown on Pi-supplemented LB medium a strong increase of the NADP⁺-dependent activity, that became predominant, occurs concurrently with a GAPDH protein increase. Our results show that B. cereus possesses two GAPDH activities, namely NAD⁺- and NADP⁺-dependent, catalyzed by two enzymes with distinct coenzyme specificity and different phosphate regulation patterns. The finding of a phosphate-stimulated NADP⁺-dependent GAPDH in B. cereus indicates that this bacterium can modulate its primary carbon metabolism according to phosphate availability.