Sulphurous mineral water oral therapy: Effects on erythrocyte metabolism

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• 作者：Maria Cristina Albertini ; Laura Teodori ; Augusto Accorsi ; Abdelaziz Soukri ; Luigi Campanella ; Francesco Baldoni ; Marina Dachà
• 关键词：Sulphurous mineral water ; Climatic idropinic treatment ; Spas ; Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ; Spring therapy
• 刊名：Food and Chemical Toxicology
• 出版年：2008
• 出版时间：October 2008
• 年：2008
• 卷：46
• 期：10
• 页码：3343-3350
• 全文大小：266 K

文摘

The ingestion of water containing hydrogen sulphide (H₂S) is common in spring sulphurous mineral water (SMW) therapy. We hypothesized that observed detrimental effects are related to the alteration of erythrocytes metabolism caused by H₂S. To verify our hypothesis, we treated 20 healthy volunteers with SMW and evidenced an increase of methemoglobin concentration, an inhibition of both erythrocyte glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate dehydrogenase (G6PDH) activities. To investigate the mechanism of H₂S effect on GAPDH activity, an in vitro study was performed by incubating both erythrocytes from 12 healthy volunteers and purified GAPDH with buffered [³⁵S]-H₂S labelled sulphurous water. The interaction between H₂S and NAD⁺(H), was also investigated. The results indicate that a direct reaction between GAPDH and H₂S does not occur and the observed decrease of GAPDH activity is to ascribe to the reaction between H₂S and NAD⁺(H). This may lead to GAPDH inhibition by two ways, namely (i) cellular NAD⁺(H) reduced availability and (ii) catalytic site blockage. In conclusion, our results show that among the detrimental effects of SMW administration are erythrocyte GAPDH and G6PDH activity inhibition and increased methemoglobin concentration. A mechanism to explain the occurrence of these biochemical events is also proposed.