Alocasia denudata Engler treatment enhance open wound healing activities in Wistar rat’s skin

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• 作者：Mazlyzam Abdul Latif ; Mohd Zulasyraf Mohd Zaki ; Tai May Leng ; Noor Hidayah Abdul Rahman ; Siti Aisyah Arshad ; Asmah Hamid ; ^{asmah0901@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; ^{asmah0901@ukm.edu.my" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Alocasia denudata Engler ; Wound healing ; Skin
• 刊名：Journal of Ethnopharmacology
• 出版年：2015
• 出版时间：24 December 2015
• 年：2015
• 卷：176
• 期：Complete
• 页码：258-267
• 全文大小：3819 K

文摘

A. denudata is traditionally used to treat various skin disorders, including wounds. It is widely used by the traditional healers as an effective wound treatment.

Aim of study

This study was done to determine A. denudata treatment effects on open wound healing activities in Wistar rat’s skin.

Materials and methods

120 Wistar rats (250–300 g) were divided into four main groups, 1.5% and 3% A. denudata stem juice treated group, 10% Solcoseryl® gel treated group as positive control and phosphate buffer saline (PBS) treated group as negative control. Six full thicknesses wounds (6 mm) were induced bilaterally on the dorsal of the rat’s skin. Rats were sacrificed on day 1, 3, 6, 10 and 14. The percentage of wound contraction, biochemical estimations, macroscopic observation and histological examinations were done to evaluate the wound healing activities.

Results

Results showed wounds treated with A. denudata stem juice possess a significant higher rate of wound contraction (p<0.001), total protein concentration (p<0.05), hexosamine concentration (p<0.001) and uronic acid concentration (p<0.001). Moreover, cathepsin B (p<0.05) and hydroxyproline (p<0.05) level showed lower concentration in wounds treated with A. denudata stem juice. Histological observation of wounds treated with A. denudata stem juice displayed organized epithelial layer with dense and compact collagen fibers.

Conclusion

Both doses of A. denudata stem juice were found to enhance wound healing process. However, wounds treated with 3% A. denudata stem juice were reported to be more effective as a wound healing agent thus support its traditional usage.