DNA sequence recognition by an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B)

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• 作者：Andrew J. Hampshire ; Hannah Khairallah ; Abedawn I. Khalaf ; Abdolrasoul H. Ebrahimabadi ; Roger D. Waigh ; Colin J. Suckling ; Tom Brown and Keith R. Fox
• 关键词：DNA recognition ; Footprinting ; Minor groove ligand
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2006
• 出版时间：1 July 2006
• 年：2006
• 卷：16
• 期：13
• 页码：3469-3474
• 全文大小：347 K

文摘

We have used DNA footprinting and fluorescence melting experiments to study the sequence specific binding of an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) to DNA. While the parent compound (thiazotropsin A) binds to the hexanucleotide sequence ACTAGT, changing one of the <i>Ni>-methylpyrrole groups to <i>Ni>-methylimidazole changes the preferred binding sequence to (A/T)CGCG(T/A). Experiments with DNA fragments that contain variants of this sequence suggest that the ligand can also bind, with lower affinity, to sequences which differ from this by 1 bp in any position.