Cell wall carbohydrates from fruit pulp of Argania spinosa: structural analysis of pectin and xyloglucan polysaccharides

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• 作者：Sophie Aboughe-Angone ; Eric Nguema-Ona ; Partha Ghosh ; Patrice Lerouge ; Tadashi Ishii ; Bimalendu Ray ; Azeddine Driouich
• 关键词：Argania spinosa ; Cell wall ; Fruit pulp ; MALDI-TOF-MS ; Pectin ; Xyloglucan
• 刊名：Carbohydrate Research
• 出版年：2008
• 出版时间：14 January 2008
• 年：2008
• 卷：343
• 期：1
• 页码：67-72
• 全文大小：170 K

文摘

Isolated cell walls of Argania spinosa fruit pulp were fractionated into their polysaccharide constituents and the resulting fractions were analysed for monosaccharide composition and chemical structure. The data reveal the presence of homogalacturonan, rhamnogalacturonan-I (RG-I) and rhamnogalacturonan-II (RG-II) in the pectic fraction. RG-I is abundant and contains high amounts of Ara and Gal, indicative of an important branching in this polysaccharide. RG-II is less abundant than RG-I and exists as a dimer. Structural characterisation of xyloglucan using enzymatic hydrolysis, gas chromatography, MALDI-TOF-MS and methylation analysis shows that XXGG, XXXG, XXLG and XLLG are the major subunit oligosaccharides in the ratio of 0.6:1:1.2:1.6. This finding demonstrates that the major neutral hemicellulosic polysaccharide is a galacto-xyloglucan. In addition, Argania fruit xyloglucan has no XUFG, a novel xyloglucan motif recently discovered in Argania leaf cell walls. Finally, the isolation and analysis of arabinogalactan-proteins showed that Argania fruit pulp is rich in these proteoglycans.