Effect of posttranslational modifications on enzyme function and assembly

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• 作者：Helena Ry拧lav谩 ; Veronika Doubnerov谩 ; Daniel Kavan ; Ond艡ej Van臎k
• 关键词：ABRF ; Association for Biomolecular Resource Facilities ; AGE ; advanced glycosylation endproduct ; ALE ; advanced lipooxidation endproduct ; AML ; acute myeloid leukemia ; APC/C ; anaphase-promoting complex/cyclosome ; CCT ; chaperone containing TCP-1 ; CDK ; cyclin-dependent kinase ; CHO ; Chinese hamster ovary ; COS-1 ; CV-1 in Origin carrying SV40 genetic material (cell line) ; CSC ; cell surface capture technology ; EC ; enzyme commission of IUPAC ; ECD ; electron capture dissociation ; EGF ; epidermal growth facto
• 刊名：Journal of Proteomics
• 出版年：30 October, 2013
• 年：2013
• 卷：92
• 期：Complete
• 页码：80-109
• 全文大小：1733 K

文摘

The detailed examination of enzyme molecules by mass spectrometry and other techniques continues to identify hundreds of distinct PTMs. Recently, global analyses of enzymes using methods of contemporary proteomics revealed widespread distribution of PTMs on many key enzymes distributed in all cellular compartments. Critically, patterns of multiple enzymatic and nonenzymatic PTMs within a single enzyme are now functionally evaluated providing a holistic picture of a macromolecule interacting with low molecular mass compounds, some of them being substrates, enzyme regulators, or activated precursors for enzymatic and nonenzymatic PTMs. Multiple PTMs within a single enzyme molecule and their mutual interplays are critical for the regulation of catalytic activity. Full understanding of this regulation will require detailed structural investigation of enzymes, their structural analogs, and their complexes. Further, proteomics is now integrated with molecular genetics, transcriptomics, and other areas leading to systems biology strategies. These allow the functional interrogation of complex enzymatic networks in their natural environment. In the future, one might envisage the use of robust high throughput analytical techniques that will be able to detect multiple PTMs on a global scale of individual proteomes from a number of carefully selected cells and cellular compartments. This article is part of a Special Issue entitled: Posttranslational Protein modifications in biology and Medicine.