A novel methodology was developed to quantify functional microorganisms in SNEDPR.
The first stoichiometric model of DGAOs via nitrite and nitrate was developed.
DGAOs conducted 64.6% of aerobic N removal and APAOs conducted 84.9% of P uptake.
N removal is improved by reducing AGAO activity and enhancing DGAO activity.
PNED reduced 7.3% aeration and 38% intracellular carbon demand.