Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses

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• 作者：Jin Chen^a ; ^b ; Fangjun Wang^a ; ^{wangfj@dicp.ac.cn} ; Zheyi Liu^a ; ^b ; Jing Liu^a ; ^b ; Yixin Zhu^c ; Yukui Zhang^a ; Hanfa Zou^a ; ¹
• 关键词：Acetonitrile ; Background interference ; Mass spectrometry ; Electrospray ionization
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：3 February 2017
• 年：2017
• 卷：1483
• 期：Complete
• 页码：101-109
• 全文大小：1956 K
• 卷排序：1483

文摘

Electrospray ionization is performed in the presence of concentrated ACN vapor. The background interferences are feasibly suppressed and the peptide signals are enhanced in ESI–MS analyses. The median MS signal intensity was improved 5 times for the 23 BSA tryptic peptides. The number of reproducibly and accurately quantified peptides could be improved 67% in six replicate label-free quantitative proteome analyses of cell line sample.