Biosensor-assisted transcriptional regulator engineering for Methylobacterium extorquens AM1 to improve mevalonate synthesis by increasing the acetyl-CoA supply

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• 作者：Wei-Fan Liang^a ; Lan-Yu Cui^a ; Jin-Yu Cui^b ; Kai-Wen Yu^c ; Song Yang^b ; Tian-Min Wang^a ; Chang-Ge Guan^a ; Chong Zhang^a ; ^{chongzhang@tsinghua.edu.cn} ; Xin-Hui Xing^a
• 关键词：Methylobacterium extorquens AM1 ; QscR regulation ; Biosensor ; Cyclic pathway ; Acetyl-CoA ; Mevalonate
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：159-168
• 全文大小：759 K
• 卷排序：39

文摘

First establishment of high throughput screening method for M. extorquens AM1. First SATRE strategy to control carbon flux in M. extorquens AM1. Up to 0.055 mol acetyl-CoA / mol methanol in fed-batch fermentation.