Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels

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• 作者：Makoto Takenaka^a ; ^b ; Ki-Seok Yoon^a ; ^b ; ^c ; Takahiro Matsumoto^a ; ^b ; ^c ; Seiji Ogo^a ; ^b ; ^c ; ^{ogo.seiji.872@m.kyushu-u.ac.jp}
• 关键词：Acetyl-CoA production ; Pyruvate ferredoxin oxidoreductase ; Encapsulation ; Immobilization ; Kinetic parameters ; Purification and characterization
• 刊名：Bioresource Technology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：227
• 期：Complete
• 页码：279-285
• 全文大小：576 K
• 卷排序：227

文摘

PFOR was anaerobically purified from Citrobacter sp. S-77. Ceramic hydroxyapatite acted as a stable matrix of PFORS77 in alginate hydrogels. Efficient acetyl-CoA production by the immobilized PFOR was achieved. The PFORS77-HA hydrogels had remarkable reusability under anaerobic conditions.