Expression and purification of histone H3 proteins containing multiple sites of lysine acetylation using nonsense suppression
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- 作者：Isaac A. Young ; Chitvan Mittal ; Michael A. Shogren-Knaak ; knaak@iastate.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Histone ; Acetylation ; Chromatin ; Nonsense suppression
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：118
- 期：Complete
- 页码：92-97
- 全文大小：541 K
文摘
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H3 histone with up to four lysine acetylation sites expressed by nonsense suppression.
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Tetra-acetylated histones purified to homogeneity with yields of 0.06 of cells.
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Histones with fewer acetylations purified to homogeneity with much greater yields.
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As expected, tetra-acetylated H3 mononucleosomes blocks nucleosome acetylation.