Electrochemically triggered release of acetylcholine from scCO₂ impregnated conductive polymer films evokes intracellular Ca^{2 +} signaling in neurotypic SH-SY5Y cells

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• 作者：Susanne Lö ; ffler ; Silke Seyock¹ ; Rolf Nybom ; Gunilla B. Jacobson² ; Agneta Richter-Dahlfors ; ^{agneta.richter.dahlfors@ki.se}
• 关键词：Organic bioelectronics ; Drug release ; On-demand delivery ; Supercritical carbon dioxide ; Acetylcholine
• 刊名：Journal of Controlled Release
• 出版年：2016
• 出版时间：10 December 2016
• 年：2016
• 卷：243
• 期：Complete
• 页码：283-290
• 全文大小：847 K
• 卷排序：243

文摘

Implantable devices for electronically triggered drug release are attractive to achieve spatial and temporal control over drug concentrations in patients. Realization of such devices is, however, associated with technical and biological challenges. Among these are containment of drug reservoirs, lack of precise control cues, as well as the charge and size of the drug. Here, we present a method for electronically triggered release of the quaternary ammonium cation acetylcholine (ACh) from an impregnated conductive polymer film. Using supercritical carbon dioxide (scCO2), a film of PEDOT/PSS (poly(3,4)-ethylenedioxythiophene doped with poly(styrenesulfonate)) is impregnated with the neurotransmitter acetylcholine. The gentle scCO2 process generated a dry, drug-impregnated surface, well suited for interaction with biological material, while maintaining normal electrochemical properties of the polymer. Electrochemical switching of impregnated PEDOT/PSS films stimulated release of ACh from the polymer matrix, likely due to swelling mediated by the influx and efflux of charged and solvated ions. Triggered release of ACh did not affect the biological activity of the drug. This was shown by real-time monitoring of intracellular Ca2 + signaling in neurotypic cells growing on the impregnated polymer surface. Collectively, scCO2 impregnation of conducting polymers offers the first one-step, dopant-independent drug impregnation process, potentially facilitating loading of both anionic and cationic drugs that can be dissolved in scCO2 on its own or by using a co-solvent. We foresee that scCO2-loaded devices for electronically triggered drug release will create novel opportunities when generating active bio-coatings, tunable for specific needs, in a variety of medical settings.