Leishmania donovani AceCS enzyme possesses a conserved leucine at 684 position (L684).
Leucine to Proline mutation affects binding of the enzyme with ATP and acetate but not CoA.
L684 is not involved in maintaining structural integrity of the enzyme.
20">L684P mutation affects acetylation of AceCS.
25">Changes in kinetic parameters upon mutation are validated by in silico modeling of enzyme.