Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H2O2 release assays
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- 作者：Satomi Miwaa ; Achim Treumannb ; Amy Bella ; Giulio Vistolic ; Glyn Nelsona ; d ; Sam Haye ; Thomas von Zglinickia ; t.vonzglinicki@ncl.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Amplex Red ; Mitochondria ; Liver ; Reactive oxygen species ; Measurement ; Carboxylesterase
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：90
- 期：Complete
- 页码：173-183
- 全文大小：3792 K
文摘
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The H2O2 probe Amplex Red crosses the mitochondrial membrane.
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Carboxylesterase catalyses HRP-independent oxidation of Amplex Red.
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This effect needs to be blocked to enable correct H2O2 measurements in many tissues.
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PMSF inhibits HRP-independent conversion of Amplex Red.