The role of N-acetyl-methioninate as a new stabilizer for albumin products

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• 作者：Makoto Anraku ; Yousuke Kouno ; Toshiya Kai ; Yasufumi Tsurusaki ; Keishi Yamasaki ; Masaki Otagiri
• 关键词：Albumin ; Sulfhydryl groups ; Calorimetry (DSC) ; Antioxidant activity ; N-Acetyl-l-methioninate ; Oxidation
• 刊名：International Journal of Pharmaceutics
• 出版年：2007
• 出版时间：1 February 2007
• 年：2007
• 卷：329
• 期：1-2
• 页码：19-24
• 全文大小：380 K

文摘

Sodium octanoate (Oct) and N-acetyl-l-tryptophanate (N-AcTrp) are widely used as stabilizers during the pasteurization of albumin products. However, N-AcTrp has a possible side effect of intracerebral disease. To provide safe and risk-free albumin products, we validated N-acetyl-methioninate (N-AcMet) as a new stabilizer for albumin products. The effect of N-AcMet on oxidation was examined using 2,2′-azobis(2-amidino-propane) dihydrochloride (AAPH) as an oxidizing agent. Carbonyl content in the presence of N-AcMet, as well as that in the presence of N-AcTrp after 24 h (name=""bbib2"">Anraku et al., 2004), was significantly decreased. The effect of AAPH on the oxidative status of 34-Cys on human serum albumin was also studied by HPLC. It was found that N-AcMet as well as N-AcTrp, has a large protective effect on the sulfhydryl group after 1 h. Further, N-AcMet was found to be a superior radical scavenger to N-AcTrp using 1,1′-diphenyl-2-picrylhydrazyl (DPPH) radicals. The thermal stabilizing role of N-AcMet manifested as an increase in denaturation temperature and calorimetric enthalpy, as determined by differential scanning calorimetry (DSC). In the present study, we suggest that use of N-AcMet in albumin preparation is safe and free of risk of side effects.