刊名:Biochemical and Biophysical Research Communications
出版年:2017
出版时间:22 January 2017
年:2017
卷:482
期:4
页码:968-974
全文大小:1754 K
卷排序:482
文摘
Crystal structures of TM0800 with and without FMN are determined. It consists of two domains: the N-terminal TIM barrel and a lid domain. FMN binds at the interface with two methionine packing on the isoalloxazine ring. Mutagenesis studies show Met276 and Ser280 are important in the enzymatic activity. Structural and biochemical studies show TM0800 is FabK, enoyl-ACP-reductase II.