Multi-day pre-clinical demonstration of glucose/galactose binding protein-based fiber optic sensor

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• 作者：Kristin Weidemaier¹ ; ^a ; Alexander Lastovich¹ ; ^a ; Steven Keith^a ; J. Bruce Pitner^a ; Mark Sistare^a ; Ross Jacobson^a ; David Kurisko ; ^a ; ^{dave_kurisko@bd.com"" rel=""nofollow}
• 关键词：Continuous glucose monitoring ; Glucose binding protein ; Fluorescent biosensor
• 刊名：Biosensors and Bioelectronics
• 出版年：2011
• 出版时间：15 June 2011
• 年：2011
• 卷：26
• 期：10
• 页码：4117-4123
• 全文大小：784 K

文摘

We report here the first pre-clinical demonstration of continuous glucose tracking by fluorophore-labeled and genetically engineered glucose/galactose binding protein (GGBP). Acrylodan-labeled GGBP was immobilized in a hydrogel matrix at the tip of a small diameter optical fiber contained in a stainless steel needle. The fiber optic biosensors were inserted subcutaneously into Yucatan and Yorkshire swine, and the sensor response to changing glucose levels was monitored at intervals over a 7-day period. Sensor mean percent error on day 7 was 16.4 ± 5.0 % using a single daily reference blood glucose value to calibrate the sensor. The GGBP sensor's susceptibility to common interferents was tested in a well-plate system using human sera. No significant interference was observed from the tested interferents except for tetracycline at the drug's maximum plasma concentration. The robust performance of the GGBP-based fiber optic sensor in swine models and resistance to interferents indicates the potential of this technology for continuous glucose monitoring in humans.