Genetic tools for identifying and manipulating fibroblasts in the mouse

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• 作者：Jessica M. Swonger ; Jocelyn S. Liu ; Malina J. Ivey ; Michelle D. Tallquist ; ^{mseidel@hawaii.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：αSMA ; α smooth muscle actin ; ACTA2 ; αV integrin ; alpha V integrin ; ADAM12 ; A Disintegrin And Metalloproteinase 12 ; ADRP ; adipocyte differentiation-related protein ; AV ; atrioventricular ; BAC ; bacterial artificial chromosome ; CCl4 ; carbon tetrachloride ; CD90 ; cluster of differentiation 90 ; Cre ; bacteriophage P1 recombinase ; DDR2 ; discoidin domain receptor tyrosine kinase 2 ; Dtr ; diptheria toxin receptor ; ER ; estrogen receptor ; FGF10 ; fibroblast growth factor 10 ; FoxD ; forkhead box D1 ; FoxJ ; forkhe
• 刊名：Differentiation
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：92
• 期：3
• 页码：66-83
• 全文大小：2444 K

文摘

The use of mouse genetic tools to track and manipulate fibroblasts has provided invaluable in vivo information regarding the activities of these cells. Recently, many new mouse strains have been described for the specific purpose of studying fibroblast behavior. Colorimetric reporter mice and lines expressing Cre are available for the study of fibroblasts in the organs prone to fibrosis, including heart, kidney, liver, lung, and skeletal muscle. In this review we summarize the current state of the models that have been used to define tissue resident fibroblast populations. While these complex genetic reagents provide unique insights into the process of fibrosis, they also require a thorough understanding of the caveats and limitations. Here, we discuss the specificity and efficiency of the available genetic models and briefly describe how they have been used to document the mechanisms of fibrosis.