Proteolytic cleavage in the S1-S2 linker of the Kv1.5 channel does not affect channel function
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- 作者：Andrew Hogan-Cann ; Wentao Li ; Jun Guo ; Tonghua Yang ; Shetuan Zhang ; shetuan.zhang@queensu.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：CHX ; Cycloheximide ; co-IP ; Co-immunoprecipitation ; hERG ; Human ether-a-go-go-related gene ; IKur ; The ultra-rapidly activating delayed rectifier potassium current ; PK ; Proteinase K
- 刊名：Biochimica et Biophysica Acta (BBA)-Biomembranes
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：1858
- 期：6
- 页码：1082-1090
- 全文大小：1690 K
文摘
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Proteinase K cleaves Kv1.5 channels at S1–S2 into two fragments.
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Co-IP experiments indicate that the N- and C-fragments do not associate.
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Proteinase K-cleavage of cell-surface channels does not affect Kv1.5 current.