Enzymatic production of protein hydrolysates from steelhead (Oncorhynchus mykiss) skin gelatin as inhibitors of dipeptidyl-peptidase IV and angiotensin-I converting enzyme
Gelatin hydrolysates with ACE and DPP-IV inhibitory activity were produced. Bioactivity was enhanced by either ultrafiltration or sequential enzyme hydrolysis. The <3 kDa fraction of hydrolysates produced by pepsin, Corolase®N or papain showed strong ACE and DPP-IV inhibition. ACE inhibitory activity of some hydrolysates improved after second hydrolysis with CorolaseN. Hydrolysis with papain followed by either ultrafiltration or CorolaseN yielded strong dual bioactivity.