Determine the alteration in the expression level and localization of ACTL7a in the induction of capacitation in mouse spermatozoa.
The Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, People's Republic of China.
ICR (Institute of Cancer Research) mice.
None.
Western blot, indirect immunostaining.
The expression of ACTL7a is upregulated via the PKA pathway and undergoes remodeling during the early period of capacitation in mouse spermatozoa.
ACTL7a is an essential component of capacitation in mouse spermatozoa. The alteration in the expression and localization of ACTL7a may be the primary biochemical event in the induction of capacitation in mouse spermatozoa.