Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold

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• 作者：Yonggen Jia^a ; ¹ ; Stefi Benjamin^b ; ¹ ; Qun Liu^c ; Yingqi Xu^b ; Sunil Kumar Dogga^a ; Jing Liu^c ; Stephen Matthews^b ; ^{s.j.matthews@imperial.ac.uk} ; Dominique Soldati-Favre^a ; ^{dominique.soldati-Favre@unige.ch}
• 关键词：Toxoplasma gondii ; Acylation ; Invasion ; Vaccine ; NMR ; Protein structure
• 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1865
• 期：2
• 页码：208-219
• 全文大小：2499 K
• 卷排序：1865

文摘

TgIMP1 localizes to the inner leaflet of plasma membrane (PM) via dual acylation. The first 11 amino acids are sufficient for PM targeting and the presence of lysine (K7) is critical. Disruption of TgIMP1 gene revealed no invasion defect or any measurable alteration in the lytic cycle of tachyzoites. Immunization with TgIMP1 DNA confers no selective protection to mice challenged with either wild type or IMP1-ko parasites. We present the solution structure of this domain determined from NMR data and describe a new protein fold not seen before.