The effect of rare codons following the ATG start codon on expression of human granulocyte-colony stimulating factor in Escherichia coli
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- 作者：Zeinab Karimia ; b ; Navid Nezafata ; b ; Manica Negahdaripoura ; b ; Aydin Berenjianc ; aydin.berenjian@waikato.ac.nz" class="auth_mail" title="E-mail the corresponding author ; Shiva Hemmatia ; b ; Younes Ghasemia ; b ; ghasemiy@sums.ac.ir" class="auth_mail" title="E-mail the corresponding author
- 关键词：CAI ; codon adaptation index (an index of codon usage bias towards the codon usage of highly expressed genes) ; hG-CSF ; human granulocyte colony stimulating factor ; MFE ; minimum free energy ; rhG-CSF ; recombinant hG-CSF ; SD ; Shine-Dalgarno
- 刊名：Protein Expression and Purification
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：114
- 期：Complete
- 页码：108-114
- 全文大小：929 K
文摘
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The N-terminus of human granulocyte-colony stimulating factor (hG-CSF) contains amino acids whose coding sequences are located in the rare codon table of E. coli.
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The effect of rare codons on hG-CSF expression level was evaluated through introducing silent mutations in the 5′-end of the coding sequence.
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The presence of rare codons following the ATG start codon increases the rhG-CSF expression in E. coli.