A switch in the mode of the sodium/calcium exchanger underlies an age-related increase in the slow afterhyperpolarization

详细信息    查看全文

• 作者：Greg Scutt^a ; ^{g.scutt@brighton.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Marcus Allen^a ; Gyö ; rgy Kemenes^b ; Mark Yeoman^a
• 关键词：Sodium calcium exchanger ; NCX ; Lymnaea stagnalis ; Aging ; Slow afterhyperpolarization ; Cerebral giant cells
• 刊名：Neurobiology of Aging
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：36
• 期：10
• 页码：2838-2849
• 全文大小：1985 K

文摘

During aging, the Ca²⁺-sensitive slow afterhyperpolarization (sAHP) of hippocampal neurons is known to increase in duration. This change has also been observed in the serotonergic cerebral giant cells (CGCs) of the pond snail Lymnaea stagnalis, but has yet to be characterized. In this article, we confirm that there is a reduction in firing rate, an increase in the duration of the sAHP, and an alteration in the strength and speed of spike frequency adaptation in the CGCs during aging, a finding that is compatible with an increase in the sAHP current. We go on to show that age-related changes in the kinetics of spike frequency adaptation are consistent with a reduction in Ca²⁺ clearance from the cell, which we confirm with Ca²⁺ imaging and pharmacological manipulation of the sodium calcium exchanger. These experiments suggest that the sodium calcium exchanger may be switching to a reverse-mode configuration in the CGCs during aging.