Quantitative proteomic analysis of Edwardsiella tarda in response to oxytetracycline stress in biofilm
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- 作者：Lina Suna ; b ; Huarong Chena ; b ; Wenxiong Lina ; b ; lwx@fjau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xiangmin Lina ; b ; xiangmin@fafu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Edwardsiella tarda ; Antibiotic resistance mechanism ; Quantitative proteomics ; Biofilm
- 刊名：Journal of Proteomics
- 出版年：2017
- 出版时间：6 January 2017
- 年：2017
- 卷：150
- 期：Complete
- 页码：141-148
- 全文大小：1245 K
文摘
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Altered proteins in Edwardsiella tarda biofilm were assessed using iTRAQ-based quantitative proteomics in oxytetracycline (OXY) stress.
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Bioinformatics analysis showed several biological processes such as ABC transport and metabolic pathways were involved in OXY adaption.
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The expression of some altered proteins and related genes were further verified by Western blotting and q-PCR analyses.
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A mutant of depletion-related genes was constructed and its subsequent susceptibility to antibiotics in biofilm was evaluated.