Pharmacological properties of the extract and some isolated compounds of Clausena lansium

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• 作者：A.C. Adebajo ; E.O. Iwalewa ; E.M. Obuotor ; G.F. Ibikunle ; N.O. Omisore ; C.O. Adewunmi ; O.O. Obaparusi ; M. Klaes ; G.E. Adetogun ; T.J. Schmidt ; E.J. Verspohl
• 关键词：Clausena lansium extract ; Furocoumarins ; Imperatorin ; 3-Formylcarbazole ; Antidiabetic activity ; Trichomonas gallinae ; Pharmacological properties
• 刊名：Journal of Ethnopharmacology
• 出版年：2009
• 出版时间：25 February 2009
• 年：2009
• 卷：122
• 期：1
• 页码：10-19
• 全文大小：1055 K

文摘

class=""h4"">Ethnopharmacological Relevance

Clausena lansium (Fool's Curry Leaf) is used for various ethnomedical conditions in some countries, including bronchitis, malaria, viral hepatitis, acute and chronic gastro-intestinal inflammation, and as a spicy substitute of the popular Curry leaf tree (Murraya koenigii).

class=""h4"">Aim of the study

This study was to evaluate the ethnomedical uses of the stem bark in inflammatory conditions, hepatotoxicity and to determine the anti-diabetic and anti-trichomonal properties of the plant.

class=""h4"">Materials and Method

Anti-trichomonal, in vivo and in vitro antidiabetic and insulin stimulating, anti-inflammatory, hepatoprotective and anti-oxidant activities using Trichomonas gallinae, glucose loaded rats and in vitro insulin secreting cell line (INS-1 cell), carrageenin-induced rat paw oedema, CCl₄-induced hepatotoxicity and DPPH scavenging ability methods respectively for the extracts and some isolates were determined.

class=""h4"">Results

A dichloromethane extract was superior over methanolic extract with respect to an anti-trichomonal activity which was measured after 24 and 48 h. The isolated compounds imperatorin and 3-formylcarbazole had the main anti-trichomonal activity (LC₅₀s of 6.0, 3.0 and 3.6, 9.7 ;c;g/mL after 24 and 48 h, respectively). Methanolic extract (100 mg/kg) induced maximum and significant (p c; 0.05) anti-hyperglycaemic activity of 15.8 % at 30 min and a 38.5 % increase in plasma insulin at 60 min, compared to control. The increase in plasma insulin after 60 min, compared to 0 min, was 62.0 % (p c; 0.05). The significant 174.6 % increase of insulin release from INS-1 cells (in vitro) at 0.1 mg/ml indicates that it mediates its antidiabetic action mainly by stimulating insulin release. Imperatorin and chalepin were the major active constituents increasing in vitro insulin release to 170.3 and 137.9 % , respectively. 100 mg/kg of the methanolic extract produced an anti-inflammatory activity after 4 h. A sedative effect was not observed. 100 and 200 mg/kg of methanolic extract administered i.p., reduced CCl4-induced hepatotoxicity firstly by 5.3 and 8.4 % reduction in phenobarbitone-sleeping time respectively, secondly by reversing the reduction in serum liver proteins by 7.0–8.8 % , serum AST, ALT and ALP activities by 27.7–107.9 % and thirdly by diminishing increased values of plasma AST, ALT and ALP activities by 13.2–83.8 % . The extract exhibited antioxidant activities.

class=""h4"">Conclusion

The hepatoprotective activity of C. lansium is partly due to its anti-oxidant and anti-inflammatory properties and confirms its folkloric use in the treatment of gastro-intestinal inflammation, bronchitis and hepatitis. In addition the use of C. lansium stem bark would be useful in diabetes and trichomoniasis.