Highly efficient plant regeneration and Agrobacterium-mediated transformation of Helianthus tuberosus L.
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- 作者：Mee-Jin Kima ; 1 ; Dong-Ju Ana ; 1 ; Ki-Beom Moona ; Hye-Sun Choa ; Sung-Ran Mina ; Jung-Hoon Sohnb ; Jae-Heung Jeona ; jeonjh@kribb.re.kr" class="auth_mail" title="E-mail the corresponding author ; Hyun-Soon Kima ; hyuns@kribb.re.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：BA ; benzyl adenine ; KIN ; kinetin ; IAA ; indole-3-acetic acid ; NAA ; naphthalene acetic acid ; PCR ; polymerase chain reaction
- 刊名：Industrial Crops & Products
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：83
- 期：Complete
- 页码：670-679
- 全文大小：2348 K
文摘
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Shoot regeneration efficiency from regenerated shoots was over 50%.
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MS medium supplemented with 1.0 mg L−1 zeatin was the best for shoot regeneration.
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The regeneration frequency was higher under dark conditions than under the 16L/8D.
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An efficient Agrobacterium-mediated transformation system was successfully achieved.
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This method will be useful for genetic improvement of H. tuberosus.