Somatic embryogenesis and plant regeneration of Capsicum baccatum L.

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• 作者：Peddaboina Venkataiah^a ; ; Pittampalli Bhanuprakash^a ; Sadhu Suman Kalyan^a ; Karampuri Subhash^b
• 关键词：BA ; 6-benzyl adenine ; 2 ; 4-D ; 2 ; 4-dichlorophenoxyacetic acid ; GA3 ; Gibberellic acid ; Kn ; kinetin ; MS ; Murashige and Skoog (1962) medium ; TDZ ; thidiazuron ; 2 ; 4 ; 5-T ; 2 ; 4 ; 5-trichlorophenoxy acetic acid ; Picloram ; 4-amino-3 ; 5 ; 6-tri chloropicolinic acid ; NAA ; α-naphthalene acetic acid ; IAA ; indole-3-acetic acid
• 刊名：Journal of Genetic Engineering and Biotechnology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：14
• 期：1
• 页码：55-60
• 全文大小：793 K

文摘

A plant regeneration protocol via somatic embryogenesis was achieved in cotyledon and leaf explants of Capsicum baccatum, when cultured on MS medium supplemented with various concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D, 0.5–5.0 mg l⁻¹) in combination with Kinetin (Kn, 0.5 mg l⁻¹) and 3% sucrose. Various stages were observed during the development of somatic embryos, including globular, heart, and torpedo-stages. Torpedo stage embryos were separated from the explants and subcultured on medium supplemented with various concentrations of different plant growth regulators for maturation. Maximum percentage (55%) of somatic embryo germination and plantlet formation was found at 1.0 mg l⁻¹ BA. Finally, about 68% of plantlets were successfully established under field conditions. The regenerated plants were morphologically normal, fertile and able to set viable seeds.