Curcumin promotes apoptosis in A549/DDP multidrug-resistant human lung adenocarcinoma cells through an miRNA signaling pathway

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• 作者：Jian Zhang ; Tao Zhang ; Xinyu Ti ; Jieran Shi ; Changgui Wu ; Xinling Ren ; Hong Yin
• 关键词：Curcumin ; Multidrug-resistant human lung adenocarcinoma cell line A549/DDP ; Apoptosis ; miR-186*
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2010
• 出版时间：13 August 2010
• 年：2010
• 卷：399
• 期：1
• 页码：1-6
• 全文大小：749 K

文摘

Curcumin extracted from the rhizomes of Curcuma longa L. has been shown to have inhibitory effects on cancers through its anti-proliferative and pro-apoptotic activities. Emerging evidence demonstrates that curcumin can overcome drug resistance to classical chemotherapies. Thus, the mechanisms underlying the anti-tumor activities of curcumin require further study. In our study, we first demonstrated that curcumin had anti-cancer effects on A549/DDP multidrug-resistant human lung adenocarcinoma cells. Further studies showed that curcumin altered miRNA expression; in particular, significantly downregulated the expression of miR-186* in A549/DDP. In addition, transfection of cells with a miR-186* inhibitor promoted A549/DDP apoptosis, and overexpression of miR-186* significantly inhibited curcumin-induced apoptosis in A549/DDP cells. These observations suggest that miR-186* may serve as a potential gene therapy target for refractory lung cancer that is sensitive to curcumin.