Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells
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- 作者：Daniel Wü ; stnera ; wuestner@bmb.sdu.dk" class="auth_mail" title="E-mail the corresponding author ; Frederik W. Lundb ; Clemens Rö ; hrlc ; Herbert Stanglc
- 关键词：ACAT ; acyl-coenzyme-A-acyl transferase ; ATP ; adenosine triphosphate ; BChol ; BODIPY-cholesterol (trade name ; TopFluor-cholesterol) ; BHK ; baby hamster kidney cells ; B-P-Chol ; pyrrole-ring linked BODIPY-cholesterol ; CHO ; Chinese hamster ovarian cells ; Chol-PEG ; polyethylene-glycol tagged cholesterol ; CTL ; cholestatrienol ; DAB ; diaminobezidine ; DHE ; dehydroergosterol ; DiI ; diindocarbocyanine ; DOPC ; dioleoylphosphatidylcholine ; DPPC ; dipalmitoylphosphatidylcholine ; EM ; electron microscopy ; ER ; endopl
- 刊名：Chemistry and Physics of Lipids
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：194
- 期：Complete
- 页码：12-28
- 全文大小：4626 K
文摘
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BODIPY-tagged analogs of cholesterol are suitable for quantitative live-cell imaging.
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BODIPY-cholesterol complements intrinsically fluorescent cholesterol analogues.
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Photobleaching and fluctuation microscopy report about dynamics of BODIPY-cholesterol.
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BODIPY-cholesterol can be also detected by superresolution and electron microscopy.
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A two-step model fits the kinetics of transport of BODIPY-cholesterol to endosomes.