Expressions of adenosine A_2A receptors in coronary arteries and peripheral blood mononuclear cells are correlated in coronary artery disease patients

详细信息    查看全文

• 作者：Vlad Gariboldi^a ; ¹ ; Donato Vairo^b ; ¹ ; Ré ; gis Guieu^b ; ^c ; ¹ ; ^{guieu.regis@orange.fr} ; Marion Marlingue^c ; Elé ; onore Ravis^d ; David Lagier^e ; Alissa Mari^b ; Elsa Thery^c ; Fré ; dé ; ric Collart^a ; Marine Gaudry^f ; Laurent Bonello^g ; Franck Paganelli^g ; Jocelyne Condo^b ; Nathalie Kipson^b ; Emmanuel Fenouillet^b ; Jean Ruf^b ; ² ; Giovanna Mottola^b ; ^c ; ²
• 关键词：Coronary artery disease ; Adenosine A2A receptor ; Ischemia
• 刊名：International Journal of Cardiology
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：230
• 期：Complete
• 页码：427-431
• 全文大小：374 K
• 卷排序：230

文摘

Altered coronary blood flow occurs in patients with coronary artery disease (CAD). Adenosine strongly impacts blood flow mostly via adenosine A2A receptor (A2AR) expressed in coronary tissues. As part of a systemic regulation of the adenosinergic system, we compared A2AR expression in situ, and on peripheral blood mononuclear cells (PBMC) in CAD patients.Methods and resultsAortic and coronary tissues, and PBMC were sampled in 20 CAD patients undergoing coronary artery bypass surgery and consecutively included. Controls were PBMC obtained from 15 healthy subjects. Expression and activity of A2AR were studied by Western blotting and cAMP measurement, respectively. A2AR expression on PBMC was lower in patients than in controls (0.83 ± 0.31 vs 1.2 ± 0.35 arbitrary units; p < 0.01), and correlated with A2AR expression in coronary and aortic tissues (Pearson's r: 0.77 and 0.59, p < 0.01, respectively). Basal and maximal cAMP productions following agonist stimulation of PBMC were significantly lower in patients than in controls (120 ± 42 vs 191 ± 65 and 360 ± 113 vs 560 ± 215 pg/106 cells, p < 0.05, respectively). In CAD patients, the increase from basal to maximal cAMP production in PBMC and aortic tissues was similar (+ 300% and + 246%, respectively).ConclusionExpression of A2AR on PBMC correlated with those measured in coronary artery and aortic tissues in CAD patients, A2AR activity of PBMC matched that observed in aorta, and A2AR expression and activity in PBMC were found reduced as compared to controls. Measuring the expression level of A2AR on PBMC represents a good tool to address in situ expression in coronary tissues of CAD patients.