We arrested the maturation of recipient iDCs by dnIKK2 gene.
dnIKK2-iDCs exhibited impaired ability to stimulate allogeneic T-cells.
dnIKK2-iDCs induced naïve recipient T-cells into CD4+CD25− T-cells.
CD4+CD25− T-cells suppressed T-cell alloreaction in an antigen-specific manner.
CD4+CD25− T-cells affected cytokine production.