Alloantigen-specific T-cell hyporesponsiveness induced by dnIKK2 gene-transfected recipient immature dendritic cells
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- 作者：Cai-bin Fana ; b ; 1 ; Yi Wangb ; c ; 1 ; Qing-ping Wangd ; Ke-lin Dub ; Duan-gai Wenb ; Jun Ouyangb ; ouyangjun99@sina.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：dnIKK2 ; dominant negative mutant of inhibitor of nuclear factor kappa-B kinase 2 ; DC ; dendritic cell ; iDCs ; immature dendritic cells ; dnIKK2-iDCs ; immature dendritic cells transfected with dnIKK2 gene ; Adv0 ; empty adenoviral vector ; Adv0-iDCs ; iDCs transfected with Adv0 ; MLR ; mixed leukocyte reaction ; FCM ; flow cytometry ; TEM ; transmission electron microscope ; ELISA ; enzyme-linked immunosorbent assay ; LW ; Lewis rat ; BN ; Brown Norway rat ; WI ; Wistar rat ; BN Ag ; Brown Norway rat antigen ; CD ; clust
- 刊名：Cellular Immunology
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：297
- 期：2
- 页码：100-107
- 全文大小：862 K
文摘
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We arrested the maturation of recipient iDCs by dnIKK2 gene.
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dnIKK2-iDCs exhibited impaired ability to stimulate allogeneic T-cells.
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dnIKK2-iDCs induced naïve recipient T-cells into CD4+CD25− T-cells.
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CD4+CD25− T-cells suppressed T-cell alloreaction in an antigen-specific manner.
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CD4+CD25− T-cells affected cytokine production.