Regional sympathetic activity in pre-hypertensive phase of spontaneously hypertensive rats

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• 作者：Cabassi ; Aderville ; Vinci ; Simonetta ; Calzolari ; Massimo ; Bruschi ; Giacomo ; Borghetti ; Alberico
• 关键词：microdialysis ; norepinephrine ; hypertension ; spontaneous hypertension ; spontaneously hypertensive rats
• 刊名：Life Sciences
• 出版年：1998
• 出版时间：February 13, 1998
• 年：1998
• 卷：62
• 期：12
• 页码：1111-1118
• 全文大小：550 K

文摘

Imbalances in central and peripheral sympathetic nervous system (SNS) activity have been observed in essential and experimental hypertension. This study was carried out in order to evaluate SNS activity in two distinct tissue types of spontaneously hypertensive rats (SHR), compared to Wistar-Kyoto normotensive (WKY) rats, in the pre-hypertensive phase (4-5 weeks of age). Interstitial concentrations of norepinephrine (NE) and other catecholamines were measured by microdialysis in striated muscle, whose tone is controlled by baroreflexes, and in the subcutaneous adipose tissue where sympathetic output mainly controls metabolism. Two groups of SHR and WKY male rats were studied, aged 4-5 weeks, with a mean body weight of 92 and 86 g respectively. Systolic blood pressure (SBP, tail-cuff) values were 113 mm Hg (SD ± 6.2) in SHR and 108 mm Hg (SD ± 7.3) in WKY rats (p=0.28,t test). Two microdialysis probes were positioned in the subcutaneous fatty tissue and in the striated muscle of the parascapular region and perfused with Ringers' solution. The dialysate was collected every thirty minutes for 3 hours and analyzed in HPLC-ED to determine the content of NE and other catecholamines. Interstitial levels of NE were higher in SH than in WKY rats in both tissues. Mean NE values from subcutaneous adipose tissue in 4-5 week-old SHR were 1162 ± 193 pg/ml compared to 496 ± 188 pg/ml in WKY rats (p<0.001, t test). Muscle tissue NE levels in SHR were 1241 ± 337 pg/ml vs. 521 ± 138 pg/ml in WKY rats (p<0.001, t test). Plasma NE concentrations (279 ± 61 pg/ml in SHR vs 246 ± 69 pg/ml in WKY P= 0.65, t test) were not significantly different between the two strains at this young age. These findings suggest SNS hyperactivity in young SHR, though still normotensive, possibly dissociated from regional components of regulation (baroreceptor control in striated muscle and metabolic control in subcutaneous adipose tissue).