Biofunctionalization via flow shear stress resistant adhesive polysaccharide, hyaluronic acid-catechol, for enhanced in vitro endothelialization

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• 作者：Hyunwoo Joo^a ; ^&dagger ; ; Eunkyoung Byun^b ; ^&dagger ; ; Mihyun Lee^b ; Yoonmi Hong^a ; Haeshin Lee^b ; ^{esoteric93@kaist.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Pilnam Kim^a ; ^{pkim@kaist.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Biomimetic material ; Mussel adhesion ; Surface modification ; Endothelial cells ; Cell adhesion ; Hyaluronic acid ; Catechol ; Microfluidics
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2016
• 出版时间：25 February 2016
• 年：2016
• 卷：34
• 期：Complete
• 页码：14-20
• 全文大小：1596 K

文摘

We report a single-step method for achieving in vitro endothelialization using a water-resistant adhesive polysaccharide, hyaluronic acid (HA). Introduction of adhesive properties is inspired by the catechol chemistry utilized in marine mussels, in which bioactive hyaluronic acid is chemically modified into hyaluronic acid-catechol (HA-catechol). Quantification of cell adhesiveness to HA-catechol coated substrate was performed with microfluidic device-based adhesion assay under shear forces. The HA-catechol exhibited enhanced ability of surface binding under flow shear force condition compared to unmodified HA. HA-catechol was able to retain the adhered endothelial cells under fluid shear stresses up to 150 dyn/cm², which is above the physiological condition in human vascular system. This is the first study demonstrating utility of the adhesive force of catechol-tethered polymers under continuous shear force environments such as microfluidic devices. The HA-catechol compound developed herein could provide a straightforward strategy for improving stability in surface retention and preventing substantial tissue damage from the fluctuating blood flow after vascular reconstructive surgery.