Clusters of neural stem/progenitor cells cultured on a soft poly(vinyl alcohol) hydrogel crosslinked by gamma irradiation
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- 作者：Hideki Mori ; Masayuki Hara ; hara@b.s.osakafu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Poly(vinyl alcohol) ; Neural stem cell ; Neurosphere ; Cell adhesion ; Gamma ray ; Hydrogel
- 刊名：Journal of Bioscience and Bioengineering
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：121
- 期：5
- 页码：584-590
- 全文大小：1715 K
文摘
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We prepared the gamma-crosslinked poly(vinylalcohol) (PVA) hydrogels.
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Neural stem/progenitor cells (NSPCs) from the fetal mouse brain was cultured on the PVA hydrogels.
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Cells adhered to the surface and then proliferated forming a cluster of cells in the DMEM/F12 medium to keep the immature undifferentiated state.
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Gamma-crosslinked PVA hydrogel is a useful substrate to amplify the NSPCs.