Absence of CC chemokine receptors 2a and 2b from human adipose lineage cells

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• 作者：Carolin Koenig^a ; Pamela Fischer-Posovszky^b ; Markus T. Rojewski^c ; Daniel Tews^b ; Hubert Schrezenmeier^c ; Martin Wabitsch^b ; Peter Gierschik^a ; Barbara Moepps^a ; ^{barbara.moepps@uni-ulm.de}
• 关键词：SGBS ; Simpson&ndash ; Golabi&ndash ; Behmel-Syndrome ; SVF ; stromal vascular fraction ; hMADS ; human multipotent adipose-derived stem cell ; BM-MSC ; bone marrow-derived mesenchymal stem cells ; MacCM ; macrophage-conditioned media
• 刊名：Molecular and Cellular Endocrinology
• 出版年：2013
• 出版时间：30 April, 2013
• 年：2013
• 卷：369
• 期：1-2
• 页码：72-85
• 全文大小：1503 K

文摘

Previous results have suggested the existence of receptors for monocyte chemoattractant protein-1 (MCP-1), CC chemokine receptors 2 (CCR2), in human adipocytes and their involvement in mediating effects of MCP-1 on adipocyte functions. However, the presence of CCR2 present on non-adipose-lineage cells of adipose tissue has not been excluded. We have used human Simpson-Golabi-Behmel-Syndrome (SGBS) preadipocytes and in-vitro-differentiated mature adipocytes to investigate the expression of CCR2 in human (pre)adipocytes. We found that the cells are devoid of CCR2 receptor protein and mRNA expression and fail to respond to treatment with all known CCR2 chemokine agonists. CCR2 is also absent from (pre)adipocytes prepared in vitro from human multipotent adipose-derived stem cells, bone-marrow-derived mesenchymal stem cells, or from primary (pre)adipocytes. Conditions mimicking proinflammatory changes in adipose tissue did not induce CCR2 receptor expression. We conclude that CCR2 is absent from human adipose lineage cells. Functional effects previously described for MCP-1 in human adipose tissue may be mediated indirectly through paracrine effects on non-adipose-lineage cells or by a (pre)adipocyte receptor for MCP-1 distinct from CCR2.