Identification of domains of the Tomato spotted wilt virus NSm protein involved in tubule formation, movement and symptomatology

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• 作者：Weimin Li ; Dennis J. Lew ; owski ; Mark E. Hilf ; Scott Adkins
• 关键词：Tospovirus ; TSWV ; Tobamovirus ; TMV ; NSm ; Tubule ; Movement protein ; Symptom
• 刊名：Virology
• 出版年：2009
• 出版时间：20 July 2009
• 年：2009
• 卷：390
• 期：1
• 页码：110-121
• 全文大小：2173 K

文摘

Deletion and alanine-substitution mutants of the Tomato spotted wilt virus NSm protein were generated to identify domains involved in tubule formation, movement and symptomatology using a heterologous Tobacco mosaic virus expression system. Two regions of NSm, G¹⁹-S¹⁵⁹ and G²⁰⁹-V²⁸³, were required for both tubule formation in protoplasts and cell-to-cell movement in plants, indicating a correlation between these activities. Three amino acid groups, D¹⁵⁴, EYKK^205–208 and EEEEE^284–288 were linked with long-distance movement in Nicotiana benthamiana. EEEEE^284–288 was essential for NSm-mediated long-distance movement, whereas D¹⁵⁴ was essential for tubule formation and cell-to-cell movement; indicating separate genetic controls for cell-to-cell and long-distance movement. The region I⁵⁷-N¹⁰⁰ was identified as the determinant of foliar necrosis in Nicotiana benthamiana, and mutagenesis of HH^93–94 greatly reduced necrosis. These findings are likely applicable to other tospovirus species, especially those within the ‘New World’ group as NSm sequences are highly conserved.