Enzymatic preparation of oligosaccharides by transglycosylation: A comparative study of glucosidases
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- 作者：Juan Mangas-Sá ; nchez ; juan.mangas@biotek.lu.se" class="auth_mail" title="E-mail the corresponding author ; Patrick Adlercreutz patrick.adlercreutz@biotek.lu.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Transglucosidation ; Hydrolysis ; Prebiotics ; Trisaccharide preparation
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：122
- 期：Complete
- 页码：51-55
- 全文大小：544 K
文摘
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A comparative study of glucosidases for the preparation of trisaccharides via transglycosylation has been conducted.
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High substrate concentrations were used to drive the reaction towards transglycosylation.
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The best results were obtained when using transglucosidase from Aspergillus niger, producing panose as major product with 69% yield after 6 h.
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Isomaltose was also obtained in 65% at longer reaction times.
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These results show the potential of glucosidases working as transglucosidases for oligosaccharide synthesis.