Simultaneous determination of 8 neurotransmitters and their metabolite levels in rat brain using liquid chromatography in tandem with mass spectrometry: Application to the murine Nrf2 model of depression
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- 作者：Aneta Wojnicza ; b ; c ; 1 ; José ; Avendañ ; o Ortizb ; 1 ; Ana I. Casasb ; e ; Andiara E. Freitasb ; d ; Manuela G. Ló ; peza ; b ; Ana Ruiz-Nuñ ; oa ; c ; anaruiznuno@yahoo.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：ACN ; acetonitrile ; AD ; adrenaline ; CV ; coefficient of variation ; DA ; dopamine ; EIC ; extraction ion chromatogram ; EMA ; European Medicine Agency ; ESI ; electrospray ionization ; FDA ; United States Food and Drug Administration ; GABA ; γ-aminobutyric acid ; Glu ; glutamic acid ; 5-HIAA ; 5-hydroxyindole-3 acetic acid ; HPLC ; high-performance liquid chromatography ; 5-HT ; 5-hydroxytryptamine ; IS ; internal standard ; LC&ndash ; MS/MS ; liquid chromatography&ndash ; tandem mass spectrometry ; LLOQ ; lower limit of quan
- 刊名：Clinica Chimica Acta
- 出版年：2016
- 出版时间：30 January 2016
- 年：2016
- 卷：453
- 期：Complete
- 页码：174-181
- 全文大小：429 K
文摘
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Liquid chromatography–mass spectrometry used to monitor neurotransmitter and metabolite levels
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Protein precipitation as sample preparation in rat brain matrix
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Method validated according to the recommendations of regulatory agencies
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Rapid quantification of neurotransmitters and metabolites to diagnose neurological diseases
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Application of the method to the murine Nrf2 depression model