Differential gene expression between inbred Roman high- (RHA-I) and low- (RLA-I) avoidance rats

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• 作者：Marta  ; Sabariego^a ;   ; ¹ ; M. José ;   ; Gó ; mez^a ;   ; ¹ ; Ignacio  ; Moró ; n^b ; Carmen  ; Torres^a ;   ; ^{mctorres@ujaen.es} ; Alberto  ; Fern&aacute ; ndez-Teruel^c ; Adolfo  ; Tobeñ ; a^c ; Toni  ; Cañ ; ete^c ; José ; A.  ; Martí ; nez-Conejero^d ; José ; A.  ; Horcajadas^e ; Francisco J.  ; Esteban^f
• 关键词：Anxiety ; Two-way avoidance acquisition ; cDNA microarray ; RT-PCR ; Gene expression ; Roman rats
• 刊名：Neuroscience Letters
• 出版年：2011
• 出版时间：31 October 2011
• 年：2011
• 卷：504
• 期：3
• 页码：265-270
• 全文大小：225 K

文摘

Microarray technology was used to explore differences in brain gene expression under basal conditions in two strains of psychogenetically selected rats which differ in anxiety/stress responses, the inbred Roman High-(RHA-I) and Roman Low-(RLA-I) Avoidance rats. Microarray analysis detected 14 up-regulated and 24 down-regulated genes in RLA-I vs. RHA-I rats functionally related to neurobiological processes. The differentially expressed genes CAMKK2, CRHBP, EPHX2, HOMER3, NDN, PRL and RPL6 were selected for microarray validation using qRT-PCR. EPHX2, CAMKK2 (both up-regulated in RLA-I vs. RHA-I rats) and HOMER3 (down-regulated in RLA-I vs. RHA-I rats) showed a similar tendency and fold-change both in microarray and RT-PCR analyses; PRL (up-regulated in RLA-I vs. RHA-I rats), CRHBP and RPL6 (both down-regulated in RLA-I vs. RHA-I animals) showed a similar tendency but a different order of magnitude of change among experiments; finally, NDN was validated neither in tendency nor in magnitude of change.